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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Topic 2: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 3: Blood Banking | 15–20% | - Transfusion practice and testing - Blood products and components - Blood group systems and immunology |
| Topic 4: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 5: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 6: Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Topic 7: Hematology | 20–25% | - Physiology and disease states - Laboratory testing and hemostasis |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
The laboratory is under the direction of a:
- A. pathologist
- B. medical assistant
- C. phlebotomist
- D. pharmacist
Correct Answer: A 🗳️
Sally is seeing her new primary care provider for the first time. When she signs in, she is asked to sign papers for the release of medical records, including her laboratory results. According to the Health Insurance Portability and Accountability Act (HIPAA), she must authorize release of records before
_______________would be permitted to receive and review her records.
- A. Her attorney
- B. All answers are correct
- C. Her husband
- D. Her insurance company
Correct Answer: B 🗳️
Enterococcus spp. are bile esculin positive and grow in 6.5% NaCl. If they grow on the 6 µg/mL vancomycin screen agar as this isolate does, it is vancomycin resistant. If there is no growth on the 6 µg/mL vancomycin screen agar, the isolate is susceptible to vancomycin. Streptococcus Group D, not Enterococcus will not grow in the 6.5% NaCl and should not be tested for vancomycin resistance. Staphylococcus epidermidis is catalase positive.
The images below represent a gram-positive, catalase-negative, non-motile coccus inoculated to bile esculin agar, 6.5% NaCl broth and to 6 µg/mL of vancomycin in Mueller Hinton agar. It should be reported as:
- A. Staphylococcus epidermidis, Resistant to vancomycin
- B. Enterococcus spp., Susceptible to vancomycin
- C. Enterococcus spp., Resistant to vancomycin
- D. Streptococcus Group D, not Enterococcus
Correct Answer: C 🗳️
Match each of the following:
1. Ratio of cellular area to total area in the bone marrow section.
2. Number of myeloid cells compared to nucleated erythroid cells.
3. Use low power to estimate their quantity and appearance.
4. Use Perls' Prussian blue stain.
- A. Myeloid-erythroid ratio
- B. Overall cellularity
- C. Megakaryocytes
- D. Stored iron
Correct Answer: A,B,C,D 🗳️
Leptin signals the hypothalamus that there are changes in fat stores.
Resistin increases insulin resistance and enhances adhesion molecules present on endothelial cells.
IL-6 responds to tissue injury. IL-6 increases insulin resistance by inhibiting insulin receptor signal transduction in liver cells. It also increases other inflammatory cytokines, interleukin-1 (IL-1) and TNF-a, and stimulates the liver to produce C-reactive protein (CRP).
Adipose tissue and liver cells produce angiotensinogen, a precursor of angiotensin II. Besides increasing blood pressure, angiotensin II may stimulate adipose cell formation and thus increase adipose mass.
Which one of the following adipocyte products is an important messenger in metabolism, signaling the hypothalamus that there are changes in fat stores?
- A. IL-6
- B. Angiotensinogen
- C. Leptin
- D. Resistin
Correct Answer: C 🗳️
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